Lumefantrine
and Glimepiride (IS) were extracted from human plasma by Precipitation followed
by Solid phase extraction using Orochem (30 mg/1 CC) solid phase extraction
cartridge. The chromatographic separation was performed on Hypurity C18 (50
cm×4.6 mm), 5 μ column. The mobile phase consisted of Acetonitrile: 2 mM
Ammonium Acetate (pH: 3.5) (90:10, % v/v) was delivered at rate of 0.600 mL/min
with Splitter. Detection and quantitation were performed by a triple quadrupole equipped with electro spray ionization and multiple reaction monitoring inpositive ionization mode (API 3000). The most intense [M-H]- transition for
Lume fantrine at m/z 528.0→510.0 and for IS at m/z 491.2→352.0 were used for
quantification.
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